Autoradiography is a technique used to visualize the distribution of radioactive substances within a sample.

DEFINITION

Autoradiography is an imaging technique that uses radioactive sources contained within the exposed sample. It produces an image on an X-ray film or nuclear emulsion by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance.

Principle

The principle of autoradiography is based on the interaction of radiation with photographic film. When a radioactive substance decays, it emits radiation that can strike silver halide crystals in the emulsion of a photographic film.

Applications

• Visualizing the distribution of radiolabeled molecules in cells and tissues. 
• Identifying radioactive contaminants in environmental samples. 

Types of autoradiography

• Contact autoradiography
• Emulsion autoradiography

Advantages:

• Autoradiography is a sensitive technique that can detect very small amounts of radioactivity.
• Autoradiography can be used to visualize the distribution of radioactive substances in a variety of materials.
• Autoradiography is a relatively inexpensive technique.

Disadvantages:

• Autoradiography is a time-consuming technique.
• Autoradiography requires the use of radioactive materials, which can pose potential health hazards.
• Autoradiography is not a quantitative technique. It can be difficult to determine the exact amount of radioactivity in a sample from an autoradiograph.