HPLC 

High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

Applications –

(i)              Pharmaceutical Industry

(ii)            Detecting Drugs in urine

(iii)          Separating the components of a complex biological sample

HPTLC 

High-performance thin layer chromatography (HPTLC) is an advanced form of thin layer chromatography (TLC). It uses the same principles as TLC, but with a number of enhancements that improve the resolution, sensitivity, and reproducibility of the technique.

-        One of the key enhancements in HPTLC is the use of high-quality TLC plates with finer particle sizes in the stationary phase. This allows for better resolution of the components in a mixture.

-        Applications –

(i)              Separating and identifying the components of a food sample

(ii)            Testing the purity of a drug sample

(iii)          Monitoring the progress of a chemical reaction

(iv)           Identifying the components in a plant extract

(v)             Quantifying the amount of a drug in a blood sample


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