Isolation & identification of atropine & Quinine Isolation & identification of Atropine Isolation: Moisten the powdered bark with an aqueous solution of sodium carbonate and extract it with ether or benzene. The free alkaloids are extracted from the solvent and acidified with acetic acid. Discard the aqueous layer. Add sodium hydroxide to the organic layer to precipitate atropine sulfate. Filter, recrystallize, and purify the crystals with activated charcoal. Dissolve the atropine sulfate in dilute sulfuric acid and make it alkaline with ammonia. Atropine will precipitate as crystals. Wash and dry them at 45-55°C. Identification: Vitali-Morin Test: Atropine + Small Amount of Con. HNO3 Evaporated to dryness in Waterbath. Residue is dissolved in 1 ml Acetone and few drops of freshly prepared Alcoholic KOH, Observation: Violet Colour (Tropane Neuclus) Isolation & identification of Quinine Isolation Here are some steps for isolating ...
LIQUORICE Biological Source Plant Part – Peeled and un peeled stolons, root and stem Scientific Name – Glycyrrhiza glabra Family – Leguminosae Morphology Odour : Characteristic Taste : Sweet Shape : Cylindrical Chemical Constituents * Saponin glycoside: Glycyrrhizin and glycyrrhizin acid * Coumarin derivatives – Umbellirerone Chemical Tests Salkowski test few drops of con. H 2 SO 4 + liquorice powder ¯ Reddish Brown colour liquorice contains glycyrrhi...
Cardiac Glycoside DEF - Cardiac glycosides are a class of organic compounds that increase the output force of the heart and decrease its rate of contractions by inhibiting the cellular sodium-potassium ATPase pump. General structure- The general structure of a cardiac glycoside consists of a steroid molecule attached to a sugar ( glycoside ) and an R group. [4] The steroid nucleus consists of four fused rings to which other functional groups such as methyl , hydroxyl , and aldehyde groups can be attached to influence the overall molecule's biological activity. [4] Cardiac glycosides also vary in the groups attached at either end of the steroid. ð All glycosides consists of an aglycone which is attached to one or more sugar (glycone) moieties. ð The Aglycone part have the pharmacological activity → Attached sugar moiety modify the solubility and Permeability. ð The Aglycone part consist of Cyclopentanope...
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