General Method OF Isolation of Glycoside Glycoside is a molecule in which a sugar (Glycone part) is attached to a Non-sugar (Aglycone) functional group via a Glycoside bond ( O/S/N/C). upon hydrolysis , glycosides are divided into a non – sugar group ( which have some pharmacological Action ) and a sugar moiety, which don’t have any pharmacological action. Example: Anthraquinone, Coumarin, Flavonoid glycosides. Isolation Sta-otto method Find me powdered drag containing glycoside is extracted by successive extraction in a soxhlet apparatus with alcohol as solvent. The thermolabile glycoside should be extracted at below 45 degree Celsius. The extract is treated with lead acetate to precipitate tannins and filtered it. To the filtrate hydrogen sulphide gas is passed, The excess of lead acetate is precipitated as lead sulphide. Pure component is determined by the spectroscopy method.
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