Explain Combined umbelliferone test

The Combined Umbelliferone test is a specific test for the presence of combined umbelliferone in a substance.

Process
  1. Triturate the drug (if in tear form) with sand.
  2. Boil 0.5 g of the drug with 3 ml of hydrochloric acid and 3 ml of water for 5 to 10 min. 
  3. Filter the mixture and to the filtrate add an equal volume of alcohol and excess of strong solution of ammonia. (Alcohol : Ammonia = 1: 1) 
Observation: A blue fluorescence is produced because of the presence of combined umbelliferone. 

This test is used in the analysis of substances like Asafoetida

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