Isolation & identification of atropine & Quinine Isolation & identification of Atropine Isolation: Moisten the powdered bark with an aqueous solution of sodium carbonate and extract it with ether or benzene. The free alkaloids are extracted from the solvent and acidified with acetic acid. Discard the aqueous layer. Add sodium hydroxide to the organic layer to precipitate atropine sulfate. Filter, recrystallize, and purify the crystals with activated charcoal. Dissolve the atropine sulfate in dilute sulfuric acid and make it alkaline with ammonia. Atropine will precipitate as crystals. Wash and dry them at 45-55°C. Identification: Vitali-Morin Test: Atropine + Small Amount of Con. HNO3 Evaporated to dryness in Waterbath. Residue is dissolved in 1 ml Acetone and few drops of freshly prepared Alcoholic KOH, Observation: Violet Colour (Tropane Neuclus) Isolation & identification of Quinine Isolation Here are some steps for isolating ...
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